Novel dendrimers containing redox mediator: Enzyme immobilization and applications
Journal of Molecular Structure, cilt.1191, ss.158-164, 2019 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 1191
- Basım Tarihi: 2019
- Doi Numarası: 10.1016/j.molstruc.2019.04.090
- Dergi Adı: Journal of Molecular Structure
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.158-164
- Anahtar Kelimeler: Dendrimer, Ferrocene, Immobilization, Glucose oxidase, Diabetes mellitus
- Kırklareli Üniversitesi Adresli: Evet
Özet
In this study, new dendrimers with a ferrocene core were produced by means of a divergent method for the immobilization of the glucose oxidase enzyme (GOx). Pt(II)and Pt(IV)ions were attached to the terminal groups of dendrimers. Metal-containing dendrimers, [Fc(MGlu)2-Aph-Pt(II)]and [Fc(MGlu)2-Aph-Pt(IV)], were obtained using the “template method” with aminophenol and Pt(II)/Pt(IV)cations. These compounds have been characterized by molar conductivity, magnetic susceptibility, FTIR, UV–Vis, 1H NMR and LC-MS methods. The GOx enzyme was immobilized on the Fc(MGlu)2, [Fc(MGlu)2-Aph-Pt(II)]and [Fc(MGlu)2-Aph-Pt(IV)]dendrimers, and the immobilized enzyme optimization parameters (substrate concentration, temperature, pH, reusability and storage capacity)were determined. Their Km (mM)and Vmax (mM.min−1)values were calculated from the Michaelis–Menten equation. The reusability of the immobilized glucose oxidase enzyme was investigated in an artificial urine medium. The research showed that [Fc(MGlu)2-Aph-Pt(II)]retains more than 61.23% of its initial activity after 10 successive cycles, which is a remarkable result.