Cysteamine-palladium complex ([Pd(μ-OAc)(ppy)]2, ppy:2-phenylpyridine, PhMe)-modified peroxidase biosensor immobilized on a gold electrode
Artificial Cells, Nanomedicine and Biotechnology, cilt.42, sa.6, ss.413-417, 2014 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 42 Sayı: 6
- Basım Tarihi: 2014
- Doi Numarası: 10.3109/21691401.2013.815193
- Dergi Adı: Artificial Cells, Nanomedicine and Biotechnology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.413-417
- Anahtar Kelimeler: biosensor, cysteamine-palladium complex, hydrogen peroxide, peroxidase
- Kırklareli Üniversitesi Adresli: Evet
Özet
A new peroxidase biosensor was developed using cysteamine-palladium complex-modified gold electrode. The principle of the measurements is based on monitoring increase in the oxidation potential of palladium complex (at + 0.47 V vs Ag/AgCl) using amperometric detection. In the optimization studies of the biosensor, effects of enzyme amount, palladium complex amount, and duration of SAM formation on biosensor responses were investigated to optimize the bioactive layer. The biosensor has a fast response time of less than 10 s to hydrogen peroxide (H2O2), with a linear range of 5.0 × 10-6 to 150 × 10-6 M and a detection limit of 3.38 × 10-6 M.