Improving catalytic hydrolysis reaction efficiency of sol-gel-encapsulated Candida rugosa lipase with magnetic β-cyclodextrin nanoparticles
Colloids and Surfaces B: Biointerfaces, cilt.113, ss.182-189, 2014 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 113
- Basım Tarihi: 2014
- Doi Numarası: 10.1016/j.colsurfb.2013.08.019
- Dergi Adı: Colloids and Surfaces B: Biointerfaces
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.182-189
- Anahtar Kelimeler: Lipase, Cyclodextrin, Fe3O4, Enantioselectivity, Drug
- Kırklareli Üniversitesi Adresli: Evet
Özet
A silica-based β-cyclodextrin was immobilized on magnetic nanoparticles to obtain a macrocyclic compound with magnetic property. Then, the β-cyclodextrin-grafted magnetic nanoparticles were encapsulated with Candida rugosa lipase in sol-gel matrices using alkoxysilane precursors. The catalytic activity of the encapsulated lipases was evaluated with model reactions, i.e., the hydrolysis of p-nitro-phenylpalmitate (p-NPP) and the enantioselective hydrolysis of rasemic Naproxen methyl ester that was studied in an aqueous buffer solution/isooctane reaction system. The results indicate that the cyclodextrin-based, encapsulated lipase particularly exhibited high conversion and enantioselectivity behavior compared to the sol-gel free lipase. It was also observed that excellent enantioselectivity (E=399) was obtained for the encapsulated lipase with magnetic β-cyclodextrin that has an ee value of S-Naproxen acid of about 98%. © 2013 Elsevier B.V.