Evaluation of Sphingolipid Metabolism in Silicosis Patients
Journal of Occupational and Environmental Medicine, cilt.68, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 68
- Basım Tarihi: 2026
- Doi Numarası: 10.1097/jom.0000000000003525
- Dergi Adı: Journal of Occupational and Environmental Medicine
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CINAHL, EMBASE, Environment Index, MEDLINE, Public Affairs Index
- Anahtar Kelimeler: silicosis, metabolomics, molecular docking, sphingolipid, sphingosine kinase, ceramidase
- Kırklareli Üniversitesi Adresli: Evet
Özet
Objective Silicosis is a fibrotic lung disease caused by the inhalation of crystalline silica. This study investigates sphingolipid metabolism in silicosis patients using plasma samples. Methods GC-MS-based untargeted metabolomics identified alterations in the sphingolipid pathway, while LC-MS targeted specific ceramide and sphingomyelin species. Enzyme activities of acid ceramidase, neutral sphingomyelinase, sphingosine kinase-1, and sphingosine-1-phosphate lyase-1 were also assessed. Results Results showed elevated sphingolipid levels and reduced enzyme activities in patients versus controls. In silico molecular docking suggested interactions between SiO and pathway enzymes. Metabolomic analysis revealed disrupted sphingolipid and fatty acid pathways. Conclusions These findings highlight the role of sphingolipid dysregulation in silicosis pathogenesis and suggest its potential as a biomarker and therapeutic target. Understanding these mechanisms could improve management and treatment strategies for this debilitating disease.