New Nanospheres to Use in the Determination of Imidan Phosmet and Vantex Pesticides
Journal of Inorganic and Organometallic Polymers and Materials, cilt.31, sa.7, ss.2915-2924, 2021 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 31 Sayı: 7
- Basım Tarihi: 2021
- Doi Numarası: 10.1007/s10904-020-01871-5
- Dergi Adı: Journal of Inorganic and Organometallic Polymers and Materials
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core, Chimica, Compendex, INSPEC
- Sayfa Sayıları: ss.2915-2924
- Anahtar Kelimeler: Acetylcholinesterase, Coordination polymer, Pesticide, Nanospheres, Schiff bases
- Kırklareli Üniversitesi Adresli: Evet
Özet
Abstract: In this study, the usability of AChE immobilized nanospheres (NSs) was investigated to identify Imidan Phosmet and Vantex pesticides. Accordingly, (2AEPS-SalBr) coded nanosphere was synthesized in result of condensation of (Tris (2 aminoethyl)amine polymer and 5-bromine-2-hydroxy benzaldehyde. Then, new nanosphere coded 2AEPS-SalBr-Pd was synthesized using the template method. The structures of the synthesized nanospheres were characterized by the FT-IR, GPC, SEM/EDX and elemental analysis. Acetylcholinesterase (AChE) enzyme was immobilized to these nanospheres for the determination of Imidan and Vantex. The optimum conditions of the immobilized-AChE enzyme, the number of reuse, storage stability, and the kinetic parameters were determined. The immobilized AChE optimum pH was 8 for both supports, and the optimum temperature was 50 °C and 70 °C, respectively. Km and Vmax values were calculated as 1.443 mM and 0.251 mM/min for (2AEPS-SalBr)-AChE (pH 8, at 50 °C), and 0.680 mM and 0.157 mM/min for (2AEPS-SalBr-Pd)-AChE (pH 8, at 70 °C). Then the analyses of Imidan Phosmet and Vantex pesticides were carried out with immobilized AChE on nanospheres. As a result of the research, it was seen that Imidan Phosmet pesticide can be determined at a lower concentration than Vantex pesticide. Graphic Abstract: [Figure not available: see fulltext.]