The association of lectin-like oxidized LDL receptor 1 (LOX-1) K167N and 3′UTR188CT polymorphisms with maternal plasma soluble LOX-1 levels and preeclampsia risk in Turkish population
Archives of Gynecology and Obstetrics, cilt.291, sa.3, ss.563-571, 2015 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 291 Sayı: 3
- Basım Tarihi: 2015
- Doi Numarası: 10.1007/s00404-014-3457-4
- Dergi Adı: Archives of Gynecology and Obstetrics
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.563-571
- Anahtar Kelimeler: Preeclampsia, Soluble lectin-like oxidized LDL receptor-1, Lectin-like oxidized LDL receptor-1 gene polymorphism, Oxidized LDL
- Kırklareli Üniversitesi Adresli: Evet
Özet
Purpose: To investigate the main effect of polymorphisms in genes involved in endothelial pathophysiological mechanisms, LOX-1 K167N and 3′UTR188CT single nucleotide polymorphisms (SNPs) in relation to preeclampsia (PE) risk and possible interactions between the gene polymorphisms and plasma oxLDL and soluble LOX-1 (sLOX-1) levels on PE in Turkish population. Methods: LOX-1 K167N and 3′UTR188CT polymorphisms were studied in 113 pregnant women with preeclampsia and 96 healthy pregnant women by the PCR–RFLP techniques. sLOX-1 and oxLDL levels were determined by enzyme-linked immunosorbent assay (ELISA) in all study subjects. Conclusions: Our findings indicate that higher plasma sLOX-1 and oxLDL concentrations, and the LOX-1 3′UTR188C>T and LOX-1 K167N gene polymorphisms were significantly associated with risk of developing preeclampsia. Plasma sLOX-1 may be a potential therapeutic target in the treatment of preeclampsia. Results: Patients having LOX-1 3′UTR188CT (OR 3.55, 95 % CI 1.89–6.67, P = 0.001) or 3′UTR188CC (OR 3.04, 95 % CI 1.25–7.38, P = 0.012) genotype had a significantly higher risk of PE than those with 3′UTR188TT genotype. Also, patients having K167N KK (OR 2.73, 95 % CI 1.33–5.61, P = 0.005) genotype had a significantly higher risk of PE than those with K167N NN genotype. LOX-1 3′UTR188TT and LOX-1 K167N NN genotype carriers were associated with significantly increased serum sLOX-1 level (P = 0.001). We further investigated the potential combined effect of these polymorphic variants on risk of PE development. According to the combined genotype analysis of LOX-1 3′UTR188TT and K167N NN polymorphisms, sLOX-1 and oxLDL levels also showed significant differences between PE patients and controls with or without combined TT/NN genotype carriers.