Determination of myricetin in medicinal plants by high-performance liquid chromatography
Instrumentation Science and Technology, cilt.43, sa.1, ss.44-52, 2015 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 43 Sayı: 1
- Basım Tarihi: 2015
- Doi Numarası: 10.1080/10739149.2014.940533
- Dergi Adı: Instrumentation Science and Technology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.44-52
- Anahtar Kelimeler: Urtica dioica L., Terebinthina chica l., myricetin, Rosa canina L., HPLC-MS, Portuca oleracea L., extraction, high-performance liquid chromatography-mass spectrometry
- Kırklareli Üniversitesi Adresli: Evet
Özet
The concentrations of myricetin in medicinal plants such as Rosa canina L. (rosa hip), Terebinthina chica L. (terebinth), Urtica dioica L. (nettle), and Portuca oleracea L. (purslane) were determined by high-performance liquid chromatography-mass spectrometry (HPLC-MS). The aglycones of myricetin were extracted using methanol-ascorbic acid- hydrochloric acid, methanol- hydrochloric acid, and methanol, separated within 5 min, and individually quantitated in the positive ionization mode using optimized conditions for HPLC-MS. Methanol-ascorbic acid-hydrochloric acid was the optimum extraction solvent. The myricetin concentration in the plants were between 3 and 58 mg kg-1, with a limit of quantification equal to 0.1 mg L-1.